CSF return is obtained, the stylet can be replaced half way into
the needle while the test tubes are obtained from the tray. Collect
1.5-2 cc. of fluid in each of 4 to 5 tubes (except when the pressure
is elevated). More CSF should be collected if a special study such as globulin
immunoelectrophoresis is required.
would be a typical grouping of tests ordered in the emergency department:
gram stain and culture (C+S) (2 cc.)
count (for comparison to Tube 1)
serum glucose analysis should be done simultaneously.
Remember the order in which the tubes are collected or prelabel
them. Be sure to tighten the test tube tops well to prevent CSF
loss during transport to the lab.