Bernard J. Jasmin, Ph.D.
Professor and Vice-Dean of Research
Ph.D. - Université de Montréal (1989)
B.Ed. - McGill University (1983)
RGN, 451 Smyth Road, Rm. 3153, Ottawa ON K1H 8M5 Phone: 562-5800 x88383 Email: jasmin@uottawa
I am interested in the cellular and molecular mechanisms involved in the assembly, maintenance and plasticity of synapses. In this context, the vertebrate neuromuscular synapse provides an ideal system with which to decipher the basic mechanisms underlying synaptogenesis, trans-synaptic regulation and cell-cell interactions. Studies in the laboratory focus particularly on: i) the molecular signals involved in the regulation of genes encoding synaptic proteins; ii) the role of cytoskeletal elements in the organization of distinct membrane and cytoplasmic domains; and iii) the plasticity of these cellular and molecular events throughout development and in the adult. To address these issues, several in vivo models leading to acute and chronic alterations in the levels of neuromuscular activation are used as well as in vitro systems involving cultures of muscle cells subjected to various experimental treatments. Molecular biological techniques such as genomic cloning, nuclear run-on, gel-shift assays, in situ hybridization, quantitative RT-PCR at the single cell level, as well as antisense and transgenic technologies are routinely used. These experiments are complemented by cell biological techniques that include immunocytochemical, histochemical and biochemical assays. In addition, we have recently begun to examine the regulation of synaptic proteins in neurons in attempts to determine whether the same regulatory mechanisms operate in different cell types.
Ljubicic, V., Miura, P., Burt, M., Lunde, J.A., Boudreau, L., Khogali, S., Lunde, J.A., Renaud, J.-M. and Jasmin, B.J. Chronic activation of AMPK evokes the slow, oxidative myogenic program and triggers beneficial adaptations in mdx mouse skeletal muscle. Hum. Mol. Genet., in press, 2011.
Clow, C. and Jasmin, B.J. Skeletal muscle-derived BDNF regulates satellite cell proliferation and skeletal muscle regeneration in vivo. Mol. Biol. Cell, 21: 2182-2190, 2010
Beauchamp, P., van der Griessen, K., Nassif, C., von Rotz, Hillock, S., Jasmin, B.J. and Gallouzi, I. Cleavage of HuR interferes with its transportin-2 mediated nuclear import and promotes muscle fiber differentiation. Cell Death and Differentiation, 17: 1588-1599, 2010.
Miura, P., Coriati, A., Bélanger, G., Lee, J., Kothary, R., Holcik, M. and Jasmin, B.J. The utrophin A 5'UTR interacts with eEF1A2 and drives expression of cap-independent translation exclusively in skeletal muscle. Hum. Mol. Genetics, 19: 1211-1220, 2010.
Corinaldi, J., Nasrallah, R., Clark, J., Paris, G., Miura, P., Jasmin, B.J. and Hebert, R.L. Troglitazone induces extracellular matrix and cytoskeleton remodelling in the M1 cortical collecting duct cell line. Am. J. Physiol. (Renal), in press, 2010.
Chopard, A., Hillock, S. and Jasmin, B.J. Molecular events and signalling pathways involved in skeletal muscle-disused atrophy and the impact of coutermeasures. J. Cell. Mol. Med., 13: 3032-3050, 2009.
Miura, P., Chakkalakal, J., Belanger, G., Tremblay, L., Hebert, R.L., Renaud, J.M. and Jasmin B.J. Treatment of mdx mice with PPARdelta agonists increase utrophin A expression and alleviate the dystrophic pathology. Hum. Mol. Genetics, 18: 4640-4649, 2009.
Chopard, A. Lecunff, M., Danger, R., Lamirault, G., Jasmin, B.J., Marini, J.F. and Leger, J.J. Large scale mRNA analysis of woman skeletal muscles during 60 days of bedrest with and without exercise and protein supplementation as countermeasures. Physiol. Genomics, 38: 291-302, 2009.
Miura, P., Andrews, M., Holcik, M. and Jasmin, B.J. Glucocorticoid treatment of skeletal muscle cells activates the utrophin A IRES. PLoS ONE, 3(6): e2309, 2008.
Xu, J., Chou, A., Bélanger, G., Radziuk, J., Jasmin, B. J., Miki, T., Seino, S., and Renaud, J.-M. K+ATP channel-deficient pancreatic cells are streptozotocin-resistant due to lower GLUT2 activity. Am. J. Physiol. (Endocrinol. Meta.), 294(2):E326-35, 2008.
Chakkalakal, J.V., Miura, P., Michel, R.N. and Jasmin, B.J. Modulation of utrophin A mRNA stability in fast vs slow skeletal muscles via an AU-rich element and calcineurin signalling. Nucleic Acid Res., 36: 826-838, 2008.
Perkins, K.J., Basu, U., Budak, M.T., Ketterer, C., Baby, S.M., Lozynska, O., Lunde, J.A., Jasmin, B.J., Rubinstein, N.A. and Khurana, T.S. ERF Silences Extrasynaptic Utrophin by N-Box-mediated Repression in Skeletal Muscle. Mol. Biol. Cell, 18: 2864-2872, 2007.
Michel, R.N., Chin, E.R., Chakkalakal, J.V., Eibl, J.K and Jasmin, B.J. Ca2+/Calmodulin-based signalling in the regulation of the muscle fibre phenotype and its therapeutic potential via modulation of utrophin and myostatin expression. Appl. Physiol. Nutr. Metab., 32: 921-929, 2007.
Deschenes-Furry, J.L., Mousavi, K., Bolognani, F., Neve, R.L., Parks, R.J., Perrone-Bizzozero, N. and Jasmin, B.J. The RNA-binding protein HuD binds to AChE mRNA in neurons and regulates its expression following axotomy. J. Neurosci., 27: 665-675, 2007.
Chakkalakal, J.V., Michel, S.A., Chin, E., Michel, R.N., and Jasmin, B.J. Targeted inhibition of calcium/calmodulin signaling exacerbates the dystrophic phenotype in mdx mouse muscle. Hum. Mol. Genet., 15: 1423-1435, 2006.
Deschênes-Furry, J., Perrone-Bizzozero, N. and Jasmin, B.J. The RNA-binding protein HuD: A master regulator of neuronal differentiation, maintenance and plasticity. BioEssays, 28: 822-833, 2006.
Mousavi, K., and Jasmin, B.J. BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic differentiation. J. Neurosci., 26: 5739-5749, 2006.
Miura, P., and Jasmin, B.J. Upregulation of utrophin as a therapy for treating Duchenne muscular dystrophy: How close are we? Trends Mol. Med., 12(3):122-9, 2006.
Bolognani, F., Tanner, D.C., Deschenes-Furry, J., Jasmin, B.J. and Perrone-Bizzozero. In vivo postranscriptional regulation of GAP-43 mRNA by overexpression of the RNA-binding protein HuD. J. Neurochem., 96: 790-801, 2006.
Deschenes-Furry, J., Angus, L.M., Belanger, G., Mwanjewe, J., and Jasmin, B.J. Role of ELAV-like RNA-binding proteins HuD and HuR in the post-transcriptional regulation of acetylcholinesterase in neurons and skeletal muscle cells. Chem. Biol. Interact., 157: 43-49, 2005.
Angus, L.M., Chakkalakal, J.V., Harrison, M.-A., Mejat, A., Megeney, L.A., Schaeffer, L., Michel, R.N., and Jasmin, B.J. Calcineurin/NFAT signalling, together with GABP and PGC-1, drives synaptic expression of the utrophin gene in skeletal muscle. Am. J. Physiol. (Cell Physiol.), 289: C908-C917, 2005.
Miura, P, Thompson, J., Chakkalakal, J.V., Holcik, M. and Jasmin, B.J. The utrophin A 5'UTR confers IRES-mediated translational control during regeneration of skeletal muscle fibers. J. Biol. Chem., 280: 32997-33005, 2005.
Stocksley, M.A., Chakkalakal, J.V., Bradford, A., Miura, P., De Repentigny, Y., Kothary, R. And Jasmin, B.J. A 1.3 kb promoter fragment confers spatial and temporal pattern of expression to utrophin A ion skeletal muscle. Neuromusc. Dis., 15: 437-449, 2005.
Chakkalakal, J.V., Thompson, J., Parks, R.J. and Jasmin, B.J. Molecular, cellular and pharmacological therapies for Duchenne/Becker muscular dystrophy. FASEB J., 19: 880-891, 2005.
Dêchenes-Furry, J.L., Bélanger, G., Parks, R.J., Perrone-Bizzozero, N. and Jasmin B.J. HuR functions to stabilize acetylcholinesterase mRNA in differentiating skeletal muscle cells. J. Biol. Chem., 280: 25361-25368, 2005.
St-Pierre, S.J.G., Chakkalakal, J.V., Kolodziejczyk, S.M., Knudson, J.C., Jasmin, B.J., and Megeney, L.A. Deflazacort treatment alleviates the dystrophic myofiber pathology by activating a calcineurin/NF-AT pathway. FASEB J., 18: 1937-1939, 2004.
Mousavi, K., Parry, D.J. and Jasmin, B.J. BDNF rescues type IIB fibers in skeletal muscle following neonatal nerve injury. Am. J. Physiol. (Cell), 287: C22-C29, 2004.
Meulenbroek, R.A., Sargent, K.L., Lunde, J.A., Jasmin, B.J. and Parks, R.J. Incorporation of a protein IX- GFP fusion into the adenovirus capsid-generation of fluorescently-labelled virions. Mol. Ther., 9: 617-624, 2004.
Bramson, J.L., Grinshtein, N., Meulenbroek, R.A., Lunde, J., Kottachi, D., Lorimer, I.A., Jasmin, B.J. and Parks, R.J. Helper-dependent adenoviral vectors containing modified fibre for improved transduction of developing and mature skeletal muscle cells. Hum. Gene Ther.,15(2):179-88, 2004.
Chakkalakal, J.V., Harrison, M.A., Chin, E., Michel, R.N. and Jasmin, B.J. Stimulation of the calcineurin/NFAT pathway attenuates the dystrophic pathology in mdx mice. Hum. Mol. Genet., 13: 379-388, 2004.
Bélanger, G., Lunde, J.A., DesGroseillers, L. and Jasmin, B.J. Expression of the RNA-binding proteins Staufen 1 and Staufen 2 at the mammalian neuromuscular junction. J. Neurochem., 86: 669-677, 2003.
Chakkalakal, J.V., Stocksley, M.A., Harrison, M.A., Angus, L.M., Deschenes-Furry, J.L., Megeney, L.A., Chin, E., Michel, R.N. and Jasmin, B.J. Expression of Utrophin A correlates with the oxidative capacity of skeletal muscle fiber types and is regulated by the calcineurin/NFAT pathway. Proc. Natl. Acad. Sci. USA, 100: 7791-7796, 2003.
Deschenes-Furry, J.L. Belanger, G., Perrone-Bizzozero, N.I. and Jasmin, B.J. HuD regulates the stability of acetylcholinesterase mRNAs during neuronal differentiation. J. Biol. Chem., 278: 510-5717, 2003.
Chakkalakal, J.V. and Jasmin, B.J. Localizing synaptic mRNAs at the neuromuscular junction: It takes more than transcription. BioEssays, 25: 25-31, 2003.
De Kerchove d'Exaerde, A., Cartaud, J., Angus, L.M., Jasmin, B.J., Changeux, J.P. and Schaeffer, L. Motor endplate malformation and alterations in gene expression consecutive to muscle-specific expression of a dominant negative version of an Ets transcription factor. EMBO Rep., 3: 1075-1081, 2002.
Jasmin, B.J., Bélanger, G., Chakkalakal, J.V., Gramolini, A.O., Lunde, J.A., Stocksley, M.A. and Thompson, J. Regulatory events controlling the expression and localization of utrophin in skeletal muscle fibers: Insights into a therapeutic strategy for Duchenne muscular dystrophy. J. Physiol. (Paris), 96: 31-42, 2002.
Gramolini, A.O., Bélanger, G., Chakkalakal, J.A., Thompson, J.T and Jasmin, B.J. Increased expression of utrophin in slow vs fast skeletal muscle involves the 3'UTR and RNA-binding proteins. Am. J. Physiol. (Cell), 281: C1300-C1309, 2001.
Gramolini, A.O., Bélanger, G. and Jasmin, B.J. Distinct regions in the 3'UTR regulate the stability and targeting of utrophin mRNAs in skeletal muscle cells. J. Cell Biol., 154:1173-1183, 2001.
Angus, L.M., Chan, R.Y.Y. and Jasmin, B.J. Intronic E- and N-boxes regulate the transient transcriptional induction of the acetylcholinesterase gene during myogenic differentiation. J. Biol. Chem., 276:17603-17609, 2001.
Newey, S., Gramolini, A.O., Wu, J., Jasmin, B.J., Davies, K.E. and Blake, D.J. A novel mechanism for modulating synaptic gene expression: Differential localization of a-dystrobrevin transcripts in skeletal muscle. Mol. Cell. Neurosci., 17: 127-140, 2001.
Boudreau-Larivière, C., Chan, R.Y.Y., Wu, J. and Jasmin, B.J. Molecular mechanisms underlying the activity-linked alterations in acetylcholinesterase mRNAs in developing versus adult rat skeletal muscles. J. Neurochem., 74: 2250-2258, 2000.
Gramolini, A.O., Wu, J. and Jasmin, B.J. Regulation and functional significance of utrophin expression at the neuromuscular synapse. Micros. Res. Tech., 49: 90-100, 2000.
Boudreau-Larivière, C., Parry, D.J. and Jasmin, B.J. Myotubes originating from single fast and slow satellite cells display similar patterns of acetylcholinesterase expression. Am. J. Physiol. (Reg. Int. Comp. Physiol.), 278: R140-R148, 2000.
Legay, C., Mankal, F.A., Massoulié, J. and Jasmin, B.J. Stability and secretion of acetylcholinesterase splice variants in skeletal muscle cells. J. Neurosci., 19: 8252-8259, 1999.
Gramolini, A.O. and Jasmin, B.J. Expression of the utrophin gene during myogenic differentiation. Nucl. Acids Res., 27: 3603-3609, 1999.
Chan, R.Y.Y. and Jasmin, B.J. Functional significance of acetylcholinesterase in hematopoietic cells. Gene Ther. Mol. Biol., 3: 1-8, 1999.
Chan, R.Y.Y., Boudreau-Larivière, C., Angus, L.A., Mankal F. and Jasmin, B.J. An intronic enhancer containing an N-box motif is essential for synapse- and tissue-specific expression of the acetylcholinesterase gene in skeletal muscle fibers. Proc. Natl. Acad. Sci. USA, 96: 4627-4632, 1999.
Boudreau-Larivière, C. and Jasmin, B.J. CGRP decreases acetylcholinesterase expression in cultured mouse myotubes. FEBS Lett., 444: 22-26, 1999.
Gramolini, A.O., Karpati, G. and Jasmin, B.J. Discordant expression of utrophin and its mRNA in human and mouse skeletal muscles. J. Neuropathol. Exp. Pathol., 58: 235-244, 1999.
Gramolini, A.O., Angus, L.A., Schaeffer, L., Burton E.A., Tinsley, J.M., Davies, K.E., Changeux, J.-P. and Jasmin, B.J. Induction of utrophin gene expression in skeletal muscle cells by heregulin: Role of the N-box motif and GABP. Proc. Natl. Acad. Sci. USA, 96: 3223-3227, 1999.
Fernandes, K., Kobayashi, N.R., Jasmin, B.J. and Tetzlaff, W. Acetylcholinesterase gene expression in axotomized rat facial motoneurons is differentially regulated by neurotrophins: correlation with trkB and trkC mRNA levels and isoforms. J. Neurosci., 18: 9936-9947, 1998.
Gramolini, A.O. and Jasmin, B.J. Molecular mechanisms and putative signalling events controlling utrophin expression in mammalian skeletal muscle fibers. Neuromusc. Dis., 8: 351-361, 1998.
Chan, R.Y.Y., Adatia F.A., Krupa, A.M. and Jasmin B.J. Increased expression of acetylcholinesterase T and R transcripts during hematopoietic differentiation is accompanied by parallel elevations in the levels of their respective molecular forms. J. Biol. Chem., 273: 9727-9733, 1998.
Camus, G., Jasmin, B.J. and Cartaud, J. Polarized sorting and vectorial delivery of the nicotinic acetylcholine receptor to the postsynaptic membrane of Torpedo electrocytes. Eur. J. Neurosci., 10: 839-852, 1998.
Decrouy, A., Renaud, J.-M., Lunde, J.A., Dickson, G. and Jasmin, B.J. Mini- and full-length dystrophin induces the recovery of nitric oxide synthase at the sarcolemma of mdx4cv skeletal muscle fibers. Gene Ther., 5: 59-64, 1998.
Gramolini, A.O., Burton, E.A., Tinsley, J.M., Ferns, M.J., Cartaud, A., Cartaud, J., Davies, K.E., Lunde, J.A. and Jasmin B.J. Muscle and neural isoforms of agrin increase utrophin expression in cultured myotubes via a transcriptional regulatory mechanism. J. Biol. Chem., 273: 736-743, 1998.
Hubatsch, D.A. and Jasmin, B.J. Passive mechanical stimulation increases acetylcholinesterase gene expression in cultured rat myotubes. Am. J. Physiol. (Cell Physiol.), 273: C2002-C2009, 1997.
Gramolini, A.O., Dennis, C.L., Tinsley, J.M., Robertson, G.S., Cartaud, J., Davies, K.E. and Jasmin, B.J. Local transcriptional control of utrophin gene expression at the neuromuscular synapse. J. Biol. Chem., 272: 8117-8120, 1997.
Decrouy, A., Renaud, J.-M., Davis, H.L., Lunde, J.A., Dickson, G. and Jasmin, B.J. Mini dystrophin gene transfer in mdxcv4 diaphragm muscle fibres increases sarcolemmal stability. Gene Ther., 4: 401-408, 1997.
Gramolini, A.O. and Jasmin, B.J. Duchenne muscular dystrophy and the neuromuscular junction: The utrophin link. BioEssays, 19: 747-750, 1997.
Boudreau-Larivière, C., Gisiger, V., Michel, R.N., Hubatsch, D.A. and Jasmin, B.J. Fast and slow skeletal muscles express a common basic profile of acetylcholinesterase molecular forms. Am. J. Physiol. (Cell Physiol.), 272: C68-C76, 1997.
Boudreau-Larivière, C., Sveistrup, H., Parry, D.J. and Jasmin, B.J. Ciliary neurotrophic factor: regulation of acetylcholinesterase in skeletal muscle and distribution of mRNA encoding its receptor in synaptic versus extrasynaptic compartments. Neurosci., 73: 613-622, 1996.
Michel, R., Campbell, R.J. and Jasmin, B.J. Regulation of succinate dehydrogenase within muscle fiber compartments by nerve-mediated activity and CNTF. Am. J. Physiol. (Reg. Physiol.), 270: R80-R85, 1996.
Jasmin, B.J., Alameddine, H., Lunde, J.A., Stetzkowski-Marden, F., Collin, H., Tinsley, J.M., Davies, K.E., Tomé, F.M.S., Parry, D.J. and Cartaud, J. Expression of utrophin and its mRNA in denervated mdx mouse muscle. FEBS Lett., 374: 393-398, 1995.
Ray, A., Kyselovic, J., Leddy, J.J., Yiggle, J.T., Jasmin, B.J. and Tuana, B.S. Regulation of dihydropyridine and ryanodine receptor gene expression in skeletal muscle: Role of nerve, protein kinase C and cAMP pathways. J. Biol. Chem., 270: 25837-25844, 1995.
Sveistrup, H., Chan, R.Y.Y. and Jasmin, B.J. Chronic enhancement of neuromuscular activity increases acetylcholinesterase gene expression in rat skeletal muscle. Am. J. Physiol. (Cell Physiol.), 269: C856-C862, 1995.
Jasmin, B.J., Antony, C., Changeux, J.P. and Cartaud, J. Nerve-dependent plasticity of the Golgi complex in skeletal muscle fibers: Compartmentalization within the subneural sarcoplasm. Eur. J. Neurosci., 7: 470-479, 1995.
Jasmin, B.J., Campbell, R.J. and Michel, R.N. Nerve-dependent regulation of succinate dehydrogenase in junctional and extrajunctional compartments of rat muscle fibers. J. Physiol. (London), 484: 155-164, 1995.
Michel, R.N., Vu, C., Tetzlaff, W. and Jasmin, B.J. Neural regulation of acetylcholinesterase mRNAs at mammalian neuromuscular synapses. J. Cell Biol., 127: 1061-1069, 1994.
Jasmin, B.J., Lee, R.K. and Rotundo, R.L. Compartmentalization of acetylcholinesterase mRNA and enzyme at the vertebrate neuromuscular junction. Neuron 11: 467-477, 1993.
Davis, H.L. and Jasmin, B.J. Direct gene transfer into mouse diaphragm. FEBS Lett. 333: 146-150, 1993.
Jasmin, B.J., Goud, B., Camus, G. and Cartaud, J. The low molecular weight GTP-binding protein Rab6p associates with distinct post-Golgi vesicles in Torpedo marmorata electrocytes. Neurosci. 49: 849-855, 1992.
Jasmin, B.J., Changeux, J.P. and Cartaud, J. Compartmentalization of cold-stable and acetylated microtubules in the subsynaptic domain of chick skeletal muscle fibre. Nature (London) 344: 673-675, 1990.
Jasmin, B.J., Cartaud, A., Ludosky, M.A., Changeux, J.P. and Cartaud, J. Asymmetric distribution of dystrophin in developing and adult Torpedo marmorata electrocyte: Evidence for its association with the acetylcholine receptor-rich membrane. Proc. Natl. Acad. Sci. USA 87: 3938-3941, 1990.
Jasmin, B.J. and Gisiger, V. Regulation by exercise of the pool of G4 acetylcholinesterase characterizing fast muscles: Opposite effect of running training in antagonist muscles. J. Neurosci. 10:1444-1454, 1990.
Jasmin, B.J., Cartaud, J., Bornens, M. and Changeux, J.P. Golgi apparatus in chick skeletal muscle: Changes in its distribution during endplate development and after denervation. Proc. Natl. Acad. Sci. USA 86: 7218-7222, 1989.