Dr. Mary-Ellen Harper

Professor

Degree:

B.A.Sc. (Guelph), Ph.D. (Ott.), Postdoc (Cambridge, UK)

Contact info:

Roger Guindon Hall, Rm. 4220
Phone:

562-5800 x 8235 (Office)
562-5800 x 8217 (Lab)

Email: mharper@uottawa.ca

Lab website: http://bioenergeticslab.com/

Research Interests:.

Mammalian Bioenergetics: Metabolic Significance and Control of Uncoupling Proteins

Energy conversion processes in mammalian cells are far from perfect. The research conducted in Dr HarperÌs laboratory focuses specifically on the metabolic pathways that affect efficiency of energy transduction processes within different cells of the body. The cellular organelle ÎchargedÌ with the responsibility of energy conversion is the mitochondrion. It is there that the oxidation of various energy substrates converges for the production of the universal energy currency of cells, ATP. The efficiency of mitochondrial ATP synthesis, or oxidative phosphorylation, varies between different tissues of the body; between different metabolic states; and between different disease states (e.g., thyroid disorders).

The overall aim of the research projects conducted in the Harper lab is to better understand the control of cellular energy transduction processes in health and disease.

In particular, Dr. HarperÌs laboratory investigates the metabolic significance and control of uncoupling proteins (UCPs). UCPs are a subfamily of the mitochondrial carrier protein family and are located in the mitochondrial inner membrane. They have been hypothesized to cause a mitochondrial proton leak, and thereby allow protons to return to the mitochondrial matrix, bypassing ATP synthase. Thus energy substrates are oxidized, and the energy is released (or "wasted") as heat, instead of being converted to ATP. The function of such seemingly wasteful processes is a major interest of the lab. The role of uncoupling proteins in protection from reactive oxygen species, and cell death, is an expanding research interest of the laboratory group, and has implications for an improved understanding of cell death and aging processes.

Dr. HarperÌs research group uses integrative approaches spanning from bioenergetic studies of isolated mitochondria, to transfected cell cultures, to transgenic mice, and to clinical investigations. Clinical projects are conducted in collaboration with Dr. Robert Dent (Ottawa Hospital), and Dr. Ruth McPherson (University of Ottawa Heart Institute). Experimental techniques include: gene transfection, transgenics and knockouts, self-referencing microelectrode technologies, metabolic control analysis, oxygen and ion-selective electrodes, real-time PCR, Western blotting, and various forms of fluorescence microscopy.

Current Laboratory Group Members:

Postdoctoral Fellows: Brianne Thrush

Graduate Students:  Brittany Beauchamp, Ghadi Antoun, Georges Kanaan, Skye McBride

Technicians: Linda Jui, Jian Xuan

Undergraduate Thesis Students: Olivier Charette

Undergraduate Research / Opportunity Program Students: Kijoo Kim

Current Research Funding:

Canadian Institutes of Health Research (CIHR),Natural Sciences and Engineering Research Council (NSERC)  

Distinctions:

  • Canadian Society for Nutritional Sciences Centrum Award, 2003
  • Laura and Arthur Colwin Endowed Summer Research Fellowship, and HB Steinbach Fellowship in Physiology, Marine Biological Laboratory, Woods Hole, Massachusetts. 2002.
  • PremierÌs Research Excellence Award, 2000.
  • Canadian Foundation for Innovation Researcher, New Opportunities Award, 1998.
  • John Charles Polanyi Prize - Physiology and Medicine. 1995.
  • Research Associateship, Girton College, University of Cambridge. 1992.
  • Natural Sciences and Engineering Research Council Postdoctoral Fellowship, 1991-3. 

Selected Publications:

  1. Ryan J. Mailloux*, Skye L. McBride* and Mary-Ellen Harper. (2013)  Unearthing the secrets of mitochondrial ROS and glutathione in bioenergetics. Trends in Biochemical Sciences. In Press. MS#: TIBS1017.
  2. Cyril Nii-Klu Adjeitey*, Ryan J. Mailloux*, Robert A. Dekemp, Mary-Ellen Harper. (2013) Mitochondrial uncoupling in skeletal muscle by UCP1 augments energy expenditure and glutathione content while mitigating ROS production. Am J Physiol Endocrinol Metab. 305(3):E405-15
  3. Céline Aguer*, Oliver Fiehn, Erin L. Seifert*, Véronic Bézaire*, John K. Meissen, Amanda Daniels*, Kyle Scott, Jean-Marc Renaud, Marta Padilla, David R. Bickel, Michael Dysart*, Sean H. Adams, and Mary-Ellen Harper. (2013) Muscle UCP3 overexpression mimics endurance training and reduces circulating biomarkers of incomplete beta-oxidation. FASEB J.  2013/234302. In Press.
  4. Stephanie Thorn, Michael H Gollob, Mary-Ellen Harper, Rob S Beanlands, Robert A deKemp and Jean N DaSilva (2013) Chronic AMPK Activity Dysregulation Produces Myocardial Insulin Resistance in the Human Arg302Gln-PRKAG2 Glycogen Storage Disease Mouse Model. Eur J Nucl Med Molec Imaging Research. In Press.
  5. A. Brianne Thrush*, Robert Dent, Ruth McPherson, and Mary-Ellen Harper. (2013) Implications of mitochondrial uncoupling in skeletal muscle in the development and treatment of obesity. FEBS J. 2013 Jun 20. In Press. PMID: 23786211
  6. Celine Aguer*, Melissa Pasqua*, Cynthia Moffat*, Michael McBurney, Karen Jardine, Renny Zhang*, Brittany Beauchamp*, Robert Dent, Ruth McPherson, and Mary-Ellen Harper. Increased antioxidant defenses in myotubes from obese non-diabetic subjects with a family history of type 2 diabetes. BBA- Molecular Basis of Disease. Accepted May 5, 2013. In Press. BBADIS-12-441.
  7. Anne C. Harasym, A. Brianne Thrush*, Mary-Ellen Harper, David C. Wright, Catherin B. Chan (2013) Enhanced glucose homeostasis in BHE/cdb rats with mutated ATP synthase. Mitochondrion. 13(4):320-329.
  8. Robert Davies, Paulina Lau, Thet Naing, Majid Nikpay, Heather Doelle, Mary-Ellen Harper, Robert Dent and Ruth McPherson (2013) A 680 kb duplication at the FTO locus in a kindred with obesity and a distinct body fat distribution. Eur. J. Genetics. doi:10.1038/ejhg.2013.63.
  9. Ryan J. Mailloux*, Jian Ying Xuan*, Brittany Beauchamp*, Linda Jui*, Marjorie Lou, and Mary-Ellen Harper Glutaredoxin-2 is required to control proton leak through uncoupling protein-3. J. Biol. Chem.288(12):8365-79.
  10. Hang Yin, Alessandra Pasut, Vahab D. Soleimani, C. Florian Bentzinger, Ghadi Antoun*, Stephanie Thorn, Patrick Seale, Pasan Fernando, Wilfred van IJcken, Frank Grosveld, Robert A. Dekemp, Robert Boushel, Mary-Ellen Harper and Michael A. Rudnicki. MicroRNA-133 Controls Brown Adipose Determination by Targeting Prdm16. Cell Metabolism. D-12-00823. Accepted Jan 2, 2013.
  11. Ryan J. Mailloux*, Accalia Fu, Christine Robson-Doucette, Emma Allister, Michael B. Wheeler, Robert Screaton, Mary-Ellen Harper. (2012) Glutathionylation state of uncoupling protein-2 and the control of glucose-stimulated insulin secretion. J Biol Chem. 2012 Oct 3. [Epub ahead of print] PMID: 23035124
  12. Aline Pfefferle*, Ryan J. Mailloux*, Mary-Ellen Harper. (2012) Glutathionylation of UCP2 sensitizes drug resistant leukemia cells to chemotherapeutics. BBA- Molecular Cell Research. BBAMCR-12-373. In Press. PMID: 23069211
  13. Ryan J. Mailloux* and Mary-Ellen Harper. (2012) Mitochondrial proticity and ROS signaling: Lessons from the uncoupling proteins. Trends in Endo and Metab. 23(9):451-8.
  14. Céline Aguer*, and Mary-Ellen Harper. (2012) Skeletal Muscle Mitochondrial Energetics in Obesity and Type 2 Diabetes Mellitus: Endocrine Aspects. Clinical Endocrinology and Metabolism. In Press. PMID: 23168281
  15. Michael P. Siegel, T Wilbur, M Mathis, E Shankland, A Trieu, Mary-Ellen Harper, Marcinek DJ. (2012) Impaired adaptability of in vivo mitochondrial energetics to acute oxidative insult in aged skeletal muscle. Mech Ageing Dev.  Aug 19. PMID: 22935551.
  16. Erin L. Seifert*, Mark Bastianelli*, Celine Aguer*, Cynthia Moffat*, Carmen Estey*, Lauren G. Koch, Steven L. Britton, Mary-Ellen Harper. (2012) Intrinsic aerobic capacity correlates with inherent mitochondrial oxidative and H2O2 emission capacities without major shifts in myosin heavy chain isoform. J Appl Physiol. Sep 20.  PMID: 22995392
  17. Narudee Kashemsant, Septimiu Bucurescu, Mary-Ellen Harper, and Catherine B. Chan. (2012) Impairment of Proinsulin Processing in ß-cells Exposed to Saturated Free Fatty Acid is Dependent on Uncoupling Protein-2 Expression. Canadian Journal of Diabetes. In Press.
  18. Carmen Estey*, Erin L. Seifert*, Céline Aguer*, Cynthia Moffat* and Mary-Ellen Harper. (2012) Calorie restriction in mice overexpressing UCP3: evidence that prior mitochondrial uncoupling alters response. Experimental Gerontol.  47(5):361-71.
  19. Erin L. Seifert*, Annabelle Z. Caron, Katy Morin, Josée Coulombe, Xiao Hong He, Karen Jardine, Danielle Dewar-Darch, Kim Boekelheide, Mary-Ellen Harper, Michael W. McBurney. (2012) SirT1 catalytic activity is required for male fertility and metabolic homeostasis in mice. FASEB J. 26(2):555-66
  20. Ryan J Mailloux* Cyril Adjeitey*, Jian Xuan* and Mary-Ellen Harper. (2012) Crucial yet divergent roles of mitochondrial redox state in skeletal muscle versus brown adipose tissue energetics. FASEB J. 26(1):363-75.
  21. Xun Zhou, Marianna Gomez-Smith, Zhaohong Qin, Philippe Duquette, Arturo Cardenas-Blanco, PS Rai, Mary-Ellen Harper, Eve C Tsai, Hymie Anisman, Hsiao-Huei Chen HH. (2012) Ablation of LMO4 in glutamatergic neurons impairs leptin control of fat metabolism. Cell Mol Life Sci. 69(5):819-28.
  22. Céline Aguer*, Daniela Gambarotta*, Ryan J. Mailloux*, Cynthia Moffat*, Robert Dent, Ruth McPherson and Mary-Ellen Harper. (2011) Galactose enhances oxidative metabolism and reveals mitochondrial dysfunction in human primary muscle cells. PLoS ONE. 6(12):e28536. I.F.4.1
  23. Ryan J Mailloux* and Mary-Ellen Harper. (2011) Uncoupling proteins and the control of mitochondrial reactive oxygen species production. Free Radical Biology and Medicine. 51(6): 1106-15.
  24. James Thackery, Jerry Radziuk, Mary-Ellen Harper, Katherine Ascah, Robert Beanlands, Jean DaSilva. (2011) Sympathetic nervous dysregulation in the absence of left ventricular dysfunction in a rat model of insulin resistance with hyperglycemia. Cardiovascular Diabetology 10(1): 75. PubMed ID: 21831292
  25. Ryan Mailloux*, Erin Seifert*, Frederic Bouillaud, Celine Aguer*, Sheila Collins, Mary-Ellen Harper (2011) Glutathionylation acts as a control switch for uncoupling proteins UCP2 and UCP3. J Biol Chem. 286(24): 21865-75.
  26. Mailloux RJ, Dumouchel T, Aguer C, Dekemp R, Beanlands R, Harper ME. Hexokinase II acts through UCP3 to suppress mitochondrial reactive oxygen species production and maintain aerobic respiration. Biochem J. 2011 437 (2): 301-11 PMID: 21554247. Free Article
  27. Mailloux RJ, Seifert EL, Bouillaud F, Aguer C, Collins S, Harper ME. Glutathionylation acts as a control switch for uncoupling proteins UCP2 and UCP3.
    J Biol Chem. 2011 Jun 17;286(24):21865-75. Epub 2011 Apr 22. PMID: 21515686
  28. Lidell ME, Seifert EL, Westergren R, Heglind M, Gowing A, Sukonina V, Arani Z, Itkonen P, Wallin S, Westberg F, Fernandez-Rodriguez J, Laakso M, Nilsson T, Peng XR, Harper ME, Enerbäck S. The adipocyte-expressed forkhead transcription factor Foxc2 regulates metabolism through altered mitochondrial function.
    Diabetes. 2011 Feb;60(2):427-35. PMID: 21270254
  29. Ghosh S, Dent R, Harper ME, Gorman SA, Stuart JS, McPherson R. Gene expression profiling in whole blood identifies distinct biological pathways associated with obesity. BMC Med Genomics. 2010 Dec 1;3:56. PMID: 21122113
  30. Mailloux RJ, Adjeitey CN, Harper ME. Genipin-induced inhibition of uncoupling protein-2 sensitizes drug-resistant cancer cells to cytotoxic agents.
    PLoS One. 2010 Oct 13;5(10):e13289. PMID: 20967268
  31. Ghosh S, Dent R, Harper ME, Stuart J, McPherson R. Blood gene expression reveal pathway differences between diet-sensitive and resistant obese subjects prior to caloric restriction. Obesity (Silver Spring). 2011 Feb;19(2):457-63. PMID: 20885385
  32. Scimè A, Soleimani VD, Bentzinger CF, Gillespie MA, Le Grand F, Grenier G, Bevilacqua L, Harper ME, Rudnicki MA. Oxidative status of muscle is determined by p107 regulation of PGC-1alpha. J Cell Biol. 2010 Aug 23;190(4):651-62.
    PMID: 20713602
  33. Gerrits MF, Ghosh S, Kavaslar N, Hill B, Tour A, Seifert EL, Beauchamp B, Gorman S, Stuart J, Dent R, McPherson R, Harper ME. Distinct skeletal muscle fiber characteristics and gene expression in diet-sensitive versus diet-resistant obesity.
    J Lipid Res. 2010 Aug;51(8):2394-404. PMID: 20332421
  34. Irrcher I, Aleyasin H, Seifert EL, Hewitt SJ, Chhabra S, Phillips M, Lutz AK, Rousseaux MW, Bevilacqua L, Jahani-Asl A, Callaghan S, MacLaurin JG, Winklhofer KF, Rizzu P, Rippstein P, Kim RH, Chen CX, Fon EA, Slack RS, Harper ME, McBride HM, Mak TW, Park DS. Hum Mol Genet. 2010 Oct 1;19(19):3734-46.  PMID: 20639397
  35. Crawford SA, Costford SR, Aguer C, Thomas SC, deKemp RA, DaSilva JN, Lafontaine D, Kendall M, Dent R, Beanlands RS, McPherson R, Harper ME. Naturally occurring R225W mutation of the gene encoding AMP-activated protein kinase (AMPK)gamma(3) results in increased oxidative capacity and glucose uptake in human primary myotubes. Diabetologia. 2010 Sep;53(9):1986-97.  PMID: 20473479
  36. Mailloux RJ, Harper ME. Glucose regulates enzymatic sources of mitochondrial NADPH in skeletal muscle cells; a novel role for glucose-6-phosphate dehydrogenase. FASEB J. 2010 Jul;24(7):2495-506.  PMID: 20228249
  37. Erin Seifert*, Carmen Estey*, Jian Xuan* and Mary-Ellen Harper. Electron transport chain-dependent and independent mechanisms of mitochondrial H202 emission during long-chain fatty acid oxidation. J. Biol. Chem. 2010 Feb. 19, 285(8):5748-58. E pub Accepted Dec 23, 2009. PMID: 20032466.
  38. Sheila Costford*, Sean Crawford*, Robert Dent, Ruth McPherson, and Mary-Ellen Harper. (2009) Increased Susceptibility to Oxidative Damage in Post-Diabetic Human Myotubes.  Diabetologia52:2405-2415.
  39. Graham Holloway, Swati Jain, Veronic Bezaire*, Xiao-Xia Han, Jan Glatz, Joost Luiken, Mary-Ellen Harper, and Arend Bonen. (2009) FAT/CD36 null mice reveal that mitochondrial FAT/CD36 is required to up-regulate mitochondrial fatty acid oxidation in contracting muscle. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology. 297(4):R960-967.
  40. Michael Greene, James T. Thackeray, Miran Kenk, Stephanie L. Thorn, Lisa Bevilacqua*, Mary-Ellen Harper, Robert S. Beanlands, and Jean DaSilva (2009) Reduced in vivo phosphodiesterase-4 response to acute noradrenaline challenge in diet-induced obese rats. Can J Physiol and Pharmacol. 87: 196-202
  41. Sheila Costford*, Shehla Chaudhry*, Mahmoud Salkhordeh* and Mary-Ellen Harper (2008). Long-Term High Fat Feeding Induces Greater Fat Storage in Mice Lacking UCP3.  Am J. Physiol: Endo & Met. 295(5):E1018-24.
  42. Erin Seifert*, Veronic Bezaire* Carmen Estey* and Mary-Ellen Harper (2008). Essential role for uncoupling protein-3 in mitochondrial adaptation to fasting but not in fatty acid oxidation or fatty acid anion export.  J Biol Chem. 283(37): 25124-31
  43. Gino Boily, Erin L. Seifert*, Lisa Bevilacqua*, Xiao Hong He, Guillaume Sabourin, Carmen Estey*, Cynthia Moffat*, Sean Crawford*, Sarah Saliba*, Karen Jardine*, Jian Xuan*, Meredith Evans*, Mary-Ellen Harper and Michael W. McBurney. (2008) SirT1 regulates energy metabolism and response to caloric restriction in mice. PLoS ONE. Mar 12;3(3):e1759. PMID: 18335035
  44. Erin L. Seifert* and Mary-Ellen Harper. (2008) Thyroid effects on mitochondrial function. Thyroid.  18(2):145-56.
  45. Mary-Ellen Harper, Katherine Green and Martin D. Brand. (2008) The efficiency of cellular energy transduction and its implications for obesity. Ann Rev Nutrition. 28:13-33.
  46. Monique C. Saleh, Zahra Fatehi-Hassanabad, Rennian Wang, Rodolpho Nino-Fong, Dorota W. Wadowska, Glenda M. Wright, Mary-Ellen Harper and Catherine B Chan. (2008) Mutated ATP synthase induces oxidative stress and impaired insulin secretion in ß-cells of female BHE/cdb rats.  Diabetes/Metabolism Research and Reviews. 24(5):392-403.
  47. Sheila Costford*, Nihan Kavaslar, Nadav Ahituv, Shehla N. Chaudhry*, Wendy S. Schackwitz, Robert Dent, Len A. Pennacchio, Ruth McPherson and Mary-Ellen Harper. (2007) Gain-of-Function R225W Mutation in Human AMPKγ3 Causing Increased Glycogen and Decreased Triglyceride in Skeletal Muscle. PLoS ONE. Sep 19; 2(9):e9032007.
  48. Sheila Costford*, Adrienne Gowing* and Mary-Ellen Harper. (2007) Mitochondrial uncoupling as a target in the treatment of obesity.  Current Opinion in Clinical Nutrition and Metabolic Care. 10(6):671-678.
  49. Andrew N. Carley, Laura Atkinson, Arend Bonen, Mary-Ellen Harper, Stanley Kunnathu, Gary D. Lopaschuk and David L. Severson. (2007) Mechanisms responsible for enhanced fatty acid utilization by perfused hearts from type 2 diabetic db/db.  Archives of Physiology and Biochemistry. 113 (2): 65-75.
  50. Sheila Costford, Erin L. Seifert, Veronic Bezaire, Martin Gerrits, Lisa Bevilacqua, Adrienne Gowing and Mary-Ellen Harper. (2007) The energetic implications of uncoupling protein-3 in skeletal muscle. J. Appl. Physiol. Nutr. Metab. In Press.
  51. Kristi Adamo, Robert Dent, Carl Langefeld, Cox M, Williams K, Carrick KM, Joan S Stuart, Scott S. Sundseth, Mary-Ellen Harper, Ruth McPherson, and Frederique Tesson. (2007) Peroxisome proliferator-activated receptor gamma 2 and acyl-CoA synthetase 5 polymorphisms influence diet response. Obesity Res. 15(5):1068-75.
  52. Veronic Bezaire, Erin Seifert and Mary-Ellen Harper. Uncoupling protein-3: Clues in a mitochondrial mystery. FASEB J. 21(2):312-24.
  53. Sheila Costford*, Shehla N. Chaudhry*, Mahmoud Salkhordeh*, Mary-Ellen Harper. (2006) Effects of the Presence, Absence andOverexpression of Uncoupling Protein-3 on Adiposit and Fuel Metabolism in Congenic Mice. Am J. Physiol Endocrinol Metab. 290(6):E1304-12.
  54. Cathy B. Chan and Mary-Ellen Harper (2006) Uncoupling proteins: Roles in insulin resistance and insulin insufficiency. Current Diabetes Reviews. 2(3):271-283.
  55. Anthony Scime, Guillaume Grenier, Michael S. Huh, Mark A. Gillespsie, Lisa Bevilacqua, Mary-Ellen Harper and MichaelA. Rudnicki (2005) Rb and p107 Regulate Preadipocyte Differentiation into White versus Brown Fat through Repression of PGC-1alpha. Cell Metaolism 2(5): 283-95.
  56. Jagdeep Sandhu, Caroline Sodja, Kevan McRae, Yan Li, Peter Ripstein, Yau-Huei Wei, Boleslaw Lach, Fay Lee, Septimiu Bucurescu*, Mary-Ellen Harper, and Marianna Sikorska, (2005) Effects of nitric oxide donors on cybrids harboring the mitochondrial myopathy, encephalopathy, lacticacidosis and stroke like episodes (MELAS) A324G mitochondrial mutation. Biochem J. 391(2): 191-202.
  57. Darcy McLellan*, Adrienne Gowing*, Martin Gerrits*, Peter Smith, Michael Wheeler and Mary-Ellen Harper (2005). Physiological increases in uncoupling protein-3 augment fatty acid oxidation and decrease reactive oxygen species production without uncoupling respiration in muscle cells. Diabetes. Aug; 54(8): 2343-50.
  58. Lisa Bevilacqua*, Jon J. Ramsey, Kevork Hagopian, Richard Weindruch and Mary-Ellen Harper. (2005) Muscle mitochondrial proton leak and reactive oxygen species production in muscle mitochondria after long-term caloric restriction. Am J Physiol: Endo & Met. 289(3): E429-438.
  59. Veronic Bezaire*, Lawrence Spriet, Shannon Campbell, Nicole Sabet*, Martin Gerrits* Arend Bonen and Mary-Ellen Harper. (2005) Constitutive overexpression of UCP3 at physiological levels increases mouse skeletal muscle capacity for fatty acid transport and oxidation. FASEB J.  19(8):977-979
  60. Daniel E Rusyniak, Stephany L.Tandy, S. K. Hekmatyar, Edward Mills, David J. Smith, Navin Bansal, Darcy MacLellan*, Mary-Ellen Harper, and Jon E. Sprague. (2005) The role of mitochondrial uncoupling in 3,4-methylene­dioxy­meth­amphetamine mediated skeletal muscle hyperthermia and rhabdomyolysis. J. Pharmacol Exp Therapeut. 313(2): 629-639
  61. Marc Riachi*, Jean Himms-Hagen, Mary-Ellen Harper (2004) Percent relative cumulative frequency (PRCF) analysis in indirect calorimetry: Application to studies of transgenic mice Can J Physiol Pharmacol. 82(12):1075-83.
  62. Mary-Ellen Harper, Lisa Bevilacqua*, Kevork Hagopian, Richard Weindruch, Jon J Ramsey. (2004) Aging, oxidative stress and mitochondrial uncoupling. Acta Physiol Scand. 182:321-331.
  63. Lisa Bevilacqua*, Jon Ramsey, Kevork Hagopian, Richard Weindruch, and Mary-Ellen Harper (2004) Effects of short- and medium-term calorie restriction on muscle mitochondrial proton leak and reactive oxygen species production. AJP: Endo Metab. 286(5):E852-61.
  64. Éric Doucet, Marjorie Pomerleau and Mary-Ellen Harper. (2004) Effects of a short-term energy restricted diet on fasting and postprandial ghrelin.  J. Clin Endocrinol Metab 89(4):1727-32.
  65. Pascal Imbeault, Marjorie Pomerleau, Mary-Ellen Harper and Éric Doucet. (2004) Unchanged fasting and postprandial adiponectin levels following a 4-day caloric restriction in young healthy men. Clin Endocrinol (Oxf). 60(4):429-33.
  66. Jon J Ramsey, Kevork Hagopian, Teresa M. Kenny, Edward K Koomson, Lisa Bevilacqua*, Richard Weindruch, and Mary-Ellen Harper. (2004) Proton leak and hydrogen peroxide production in liver mitochondria from energy restricted rats. AJP: Reg Integr. 286: E31-40.
  67. Xiaotuan Liu, Martin Rossmeisel, Jennifer McClaine, Marc Riachi*, Mary-Ellen Harper and Leslie P. Kozak.  (2003) Paradoxical resistance to diet-induced obesity in UCP1-deficient mice. J. Clin. Invest. 111:399-407. Erratum: 111:759.
  68. Mary-Ellen Harper, Andreas Antoniou* Elizabeth Villalobos-Menuey, Alicia Russo, Richard Trauger, Minda Vendemelio, Azhar Shaikh* Jami Kupperman, Evan W. Newell, Ivan A. Bespalov, Susan S. Wallace, Ye Liu, Jeffrey Rogers, Gregory Gibbs, Sandra Berry-Lowe, Jack L. Leahy, Robert Melamede, and M. Karen Newell (2002).  Characterization of a Novel Metabolic Strategy Used by Drug Resistant Tumor Cells. FASEB J. 16:1550-1557.
  69. Mary-Ellen Harper,  Robert Dent, Shadi Monemdjou*, VJronic BJzaire*, Lloyd VanWyck, George Wells, Gul Nihan Kavaslar, Andre Gauthier, FrJdJrique Tesson and Ruth McPherson (2002)  Decreased mitochondrial proton leak and reduced expression of Ucp3 in skeletal muscle of obese diet-resistant women.  Diabetes. 51: 2459-2466.
  70. George Argyropoulos and Mary-Ellen Harper (2002)  Uncoupling proteins and thermoregulation, J. Appl. Physiol. 92: 2187-2198.
  71. Veronic Bezaire*, Wolfgang Hofmann, John K.G.Kramer, Leslie P. Kozak and Mary-Ellen Harper. (2001) Effects of fasting on muscle mitochondrial energetics and fatty acid metabolism in Ucp3 -/- and wild-type mice Am. J. Physiol,. 281(5):E975-82.
  72. Mary-Ellen Harper and Jean Himms-Hagen (2001) Mitochondrial efficiency: Lessons learned from transgenic mice. Biochim. Biophys. Acta: Bioenergetics. 1504: 159-172.    
  73. Jean Himms-Hagen and Mary-Ellen Harper (2001) Physiological role of UCP3 may be export of fatty acids from mitochondria when fatty acid oxidation predominates.  Exp. Biol. Med. 226:78-84.
  74. Kyoko Tsukiyama-Kohara, Francis Poulin, Michinori Kohara, Christine DeMaria, Alan Cheng, Anne-Claude Gingras, Asao Katsume, Mounib Elchebly, Mary-Ellen Harper, Michel Tremblay and Nahum Sonenburg (2001) Decreased adipose tissue and increased metabolism in mice lacking the translational inhibitor 4EBP1. Nature Medicine.  7:1-5.  
  75. Wolfgang E. Hofmann, Xiaotuan Liu, Christie M. Bearden, Mary-Ellen Harper, and Leslie P. Kozak (2001) Effects of genetic background on thermoregulation and fatty acid induced uncoupling of mitochondria in UCP1-deficient mice. J. Biol. Chem. 276(15):12460-12465.
  76. Shadi Monemdjou*, Wolfgang E. Hofmann, Leslie P. Kozak and Mary-Ellen Harper (2000) Increased mitochondrial proton leak in skeletal muscle mitochondria of UCP1-deficient mice. Am. J. Physiol.: Endocrinol. Metab. 279:E941-E946.
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