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Albert Stolow

Principal Research Officer, Steacie Institute for Molecular Sciences, National Research Council of Canada

Program Leader, Molecular Photonics Group, Steacie Institute for Molecular Sciences, National Research Council of Canada

Adjunct Professor
Dept. of Physics, University of Ottawa
Dept. of Chemistry, Queen's University
Dept. of Physics, Queen's University

Tel: 613 993-7388 (office)
Email: Albert.Stolow@nrc.ca
Personal web site: Queen's University
CARS Microscopy User Facility: CARSLab


Albert Stolow
Adjunct Professor

Degrees:

B.Sc. Chemistry & Physics, Queen's 1982
Ph.D. Physical Chemistry, University of Toronto 1988
NSERC Postdoctoral Fellow, University of California Berkeley 1989-1992

Albert Stolow studied Chemistry and Physics at Queen’s University and then obtained his Ph.D. degree in Physical Chemistry from the University of Toronto in 1988, studying under Nobel Laureate John C. Polanyi. Stolow was an NSERC post-doctoral fellow at the University of California, Berkeley from 1989-1992 where he worked with Nobel Laureate Yuan T. Lee. In fall 1992, Stolow joined the National Research Council where he established laboratories and research programs for the study of femtosecond molecular dynamics and quantum control. Stolow is a Fellow of both the American Physical Society and the Optical Society of America, has won several prizes, including the Keith Laidler Award of the Chemical Society of Canada, and sits on the editorial boards of various international journals. Stolow is one of only two Canadian members of the Editorial Board of the internationally known Journal of Biophotonics.

Research Interests:

  • Modern nonlinear optical microscopy.
  • Femtosecond laser physics and applications.
  • Ultrafast molecular dynamics.
  • Quantum Optical Control with applications in live cell/tissue microscopy.
  • Collaborative studies on models for disease and their molecular basis.

Major Research Interest:

Our lab's research is in the area of nonlinear optical microscopy where we are developing and applying new methods in label-free, chemical-specific microscopies. In particular, we focus on multi-modal Coherent Anti-Stokes Raman Scattering (CARS) Microscopy. CARS is analogous to Raman Microscopy but is about 100,000 times more sensitive. Our system can simultaenously record CARS, two-photon fluorescence and second ahrmonic generation microscope images. We can record real-time movies ( 2 frames/sec) of live cells and tissues without the use of any exogenous dyes or stains. Our CARS technology was recent commercialized by Olympus Corporation, producing the world's first commercially available CARS microscope. In addition, NRC and Olympus jointly created the world's first CARS Microscopy User Facility, called "CARSLab", right in downtown Ottawa. For further details: CARSLab. Current applications includes studies of hepatitis, atherosclerosis, Alzheimer's Disease, cartilage injury, vascular disease and regenerative medicine.

Selected Publications:

A.D. Slepkov, A. Ridsdale, H-N Wan, M-H Wang, A.F. Pegoraro, D.J. Moffatt, J.P. Pezacki, F-J Kao, A. Stolow Forward-Collected FLIM-CARS Microscopy Journal of Biomedical Optics (in press 2010)

A. Pegoraro, A. Slepkov, A. Ridsdale, J.P. Pezacki & A. Stolow Single laser source for multimodal CARS microscopy Applied Optics (in press 2010)

C. Weeraman, S.A. Mitchell, R. Lausten, L.J. Johnston, A. Stolow Vibrational sum frequency generation spectroscopy using inverted visible pulses Optics Express 18, 11487 (2010)

A.C.T. Ko, M.S.D. Smith, L.B. Mostaco-Guidolin, M.D. Hewko, E.K. Kohlenberg, B. Schattka, M.G. Sowa, A. Ridsdale, A.F. Pegoraro, A. Stolow, M. Shiomi Multimodal nonlinear optical imaging of atherosclerotic plaque development in myocardial infarction prone rabbits Journal of Biomedical Optics 15, 20501 (2010)

M.G. Sowa, L.B. Mostaco-Guidolin, M.S.D. Smith, E.K. Kohlenberg, A. Ridsdale, A. Stolow & A.C.T. Ko Nonlinear Optical Measurements of the Artery Wall: Parameters Related to the Progression of Atherosclerosis Measurement Science Review 9, 93 (2009)

A.F. Pegoraro, A. Ridsdale, D.J. Moffatt, J.P. Pezacki, A. Stolow, B.K. Thomas, L. Fu, L. Dong, M.E. Fermann All-fiber CARS microscopy of live cells Optics Express 17, 20700 (2009)

A.F. Pegoraro, A. Stolow, A. Ridsdale, D.J. Moffatt, J.P. Pezacki, Y. Jia CARS Microscopy Made Simple Biophotonics 18(8), 36 (2009)

R.K. Lyn, D.C. Kennedy, S.M. Sagan, D.R. Blais, Y. Rouleau, A.F. Pegoraro, X.S. Xie, A. Stolow, J.P. Pezacki Direct imaging of the disruption of hepatitis C virus replication complexes by inhibitors of lipid metabolism Virology 394, 130 (2009)

A.F. Pegoraro, A. Ridsdale, D.J. Moffatt, J.P. Pezacki, A. Stolow CARS Microscopy Made Simple Photons 7, 50 (2009)

A.F. Pegoraro, A. Ridsdale, D.J. Moffatt, Y. Jia, J.P. Pezacki & A. Stolow Optimally chirped multimodal CARS microscopy based on a single Ti:sapphire oscillator Optics Express 17, 2984 (2009)

B.J. Sussman, R. Lausten & A. Stolow Focusing of light following a 4-f pulse shaper: Considerations for quantum control Physical Review A 77 , 043416 (2008)

X-L. Nan, A.M. Tonary, A. Stolow, X.S. Xie, J.P. Pezacki Intracellular imaging of HCV RNA and cellular lipids by using simultaneous two-photon fluorescence and coherent anti-Stokes Raman scattering microscopies ChemBioChem 7 1895 (2006)

Selected Invited Lectures And Presentations:

Sigma Xi Lecture, Ottawa, ON, 17 February, 2010 Shining New Light on Live Cells: Nonlinear Optical Microscopy.

Department of Chemistry, Stanford University, Stanford, CA, USA 4 December, 2009 CARS Microscopy Made Simple.

ACS 238th National Meeting. Symposium on Biological Applications of Nonlinear Optical Imaging and Spectroscopy Washington DC, USA. 19 August 2009 Optimally chirped multimodal CARS Microscopy based on a single Ti:Sa oscilliator.

XIth Cross Border Workshop on Laser Science Ottawa, ON 29 May 2009 Optimally Chirped High Performance CARS Microscopy of Live Cells and Tissues.

Photonics North. Symposium on Photonics Design & Simulation. Quebec City, QC 24 May 2009 High Performance Multimodal CARS Microscopy using a Single Femtosecond Source.

NATO Advanced Study Institute. Laser Control & Monitoring in New Materials, Biomedicine, Environment, Security & Defense Ottawa, Canada. Nov.25 & 26, 2008 CARS Microscopy in Biomedicine: Non-linear Optics & Coherent Control.

ACFAS Symposium on Biophotonics Quebec City, Quebec. 6 May 2008 Coherent Anti-Stokes Raman Scattering (CARS) Microscopy of Live Cells.

American Physical Society. March Meeting. Focus Session on Quantum Control New Orleans, LA. USA 12 March 2008 Non-resonant, non-perturbative Dynamic Stark Control of Quantum Dynamics.

American Physical Society Meeting, Division of Atomic, Molecular & Optical Physics. Calgary, Alberta 5 – 9 June 2007 Non-perturbative Quantum Control via the Non-resonant Dynamic Stark Effect

Physics of Quantum Electronics Conference Snowbird, Utah, USA 5 January, 2007 Quantum control via the Non-Resonant Dynamic Stark Effect

Colloquium Max-Planck-Institute for Quantum Optics Garching, Germany 20 June 2006 The Three Pillars of Femtosecond Molecular Science.

Biophysical Chemistry Symposium McGill University 16 May 2006 Dynamical Aspects of DNA Photonics.

Department of Physics University of Ottawa 9 March 2006 Three Pillars of Femtosecond Science: Time, Phase, Intensity.